Hipure CFDNA Column Set
Quick Detail
- Minimum order:1000
- Place of Origin:China (Mainland)
- FOB Price : USD $23.00 / Piece
Specifications
AG-CFDNA Column Set provides a simple and reliable method for the rapid preparation of high-quality circulating cell-free DNA from serum, plasma, amniotic fluid, cerebrospinal fluid (CSF), and saliva. A combination of chemical and enzymatic methods are used to efficiently recover total DNA (including cell-free apoptotic, necrotic, mitochondrial and viral DNA) linearly from up to 10 ml of sample (Reference Figure A).
The AG-CFDNA Column Set contains with
20ml Funnel Column
CFDNA Mini Column
Collection Tube III
50ml Centrifuge Tube
| DNA Recovery | Recover DNA ≥ 100bp (optimized for recovery of cell-free DNA). Yields can vary considerably among different individuals. Typically DNA recovery ranges from 1-100 ng/ml of plasma or serum. The yield varies depending on the sample source and the health of the donor. |
| Equipment | Water bath or heat block (55ºC), microcentrifuge, vacuum/vacuum manifold or swinging bucket centrifuge. |
| Sample Sources | Serum, plasma, amniotic fluid, and cerebrospinal fluid (CSF). |
| DNA Purity | High-quality DNA is ready for all sensitive downstream applications such as qPCR and Next-Generation sequencing. |
| Storage | Eluted DNA should be stored at ≤ -20ºC. |
| Recovery Volume | ≥ 35 μl ofDNA Elution Bufferor DNase free water. |
| Processing Volume |
Plasma – Single centrifugation: up to 3 ml Plasma – Double centrifugation: up to 10 ml Serum/Amniotic fluid/Cerebrospinal fluid (CSF) – up to 10 ml Saliva – up to 1 ml Cell-free Saliva – up to 5 ml |
