Caspase 13 FLICA Detection Kit
Description
| ICT's FLICA caspase 13 detection assay is a simple yet accurate method to measure caspase 13 activity |
Product Details
ICT's caspase 13 inhibitor probe is comprised of 3 segments:
a green fluorescent label, FAM (carboxyfluorescein)
an inhibitor peptide sequence targeted by active caspase 13 (LEED)
and a fluoromethylketone group (FMK), which forms a covalent bond with the active enzyme.
FLICA kits have been used successfully with a multitude of cell lines from a variety of species (human, mouse, rat, etc), including, but not limited to, cancer cells, neurons, primary cells,
epithelial cells, endothelial cells, and macrophages. For in vitro apoptosis detection via active poly caspases, see our FLICA Poly Caspases Detection Kits
The FLICA assay also works with adherent cells and very thin tissue sections. FLICA will not work in previously fixed cells or paraffin cells, as those treatments inactivate the caspase 13 enzyme. Please call for details or technical questions at 800-829-3194.
ICT's FLICA caspase 13 detection assay is a simple yet accurate method to measure caspase 13 activity in whole cells. Simply add the FLICA caspase 13 reagent to the media and incubate. Cells
containing active caspase 13 will fluoresce green. The kit contains:
ICT's FLICA caspase 13 detection probe, FAM-LEED-FMK
10x apoptosis wash buffer
Hoechst stain (a blue DNA stain)
Propidium iodide (a red live/dead stain)
Fixative (for use after labeling with FLICA)
Trial size, ~25 tests:
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Regular size, ~100 tests:
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Caspase 13 activity may be detected in individual cells or suspensions of up to 1x10^6 cells/mL. The noncytotoxic FLICA probe is cell permeant: no lysis or permeabilization steps are necessary.
Just culture cells, add FLICA to the media, incubate, and wash. Active caspase 13 enzymes will bind to FLICA and retain it within the cells, thereby capturing the green fluorescent signal. FLICA
constantly fluoresces, therefore any unbound reagent must be washed out of the cell to remove any background noise. The resulting positive fluorescent signal can be detected with a fluorescence
microscope, a fluorescence plate reader, or a flow cytometer. The green FLICA probe, FAM, excites at 490nm and emits at 520nm.
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